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Metabolomics is the comprehensive analysis of small molecule metabolites produced by normal or abnormal cellular processes. It is fast gaining ground as a powerful tool for biomarker discovery and as an important component of systems biology research, complementing genomics, transcriptomics, and proteomics. Recent advances in LC-MS based metabolomics have created the potential to measure the levels of numerous metabolites in various media ranging from cell cultures to human biological fluids such as urine, saliva, and blood.